Evaluation of the Antioxidative and Qualitative Properties of the Tinospora cordifolia

Abstract

Background: Tinospora cordifolia is a perennial shrubby creeper, a member of the Menispermaceae family. It is a plant containing various phytochemical compounds and is used in traditional medicine. Materials and Methods: In the following study the qualitative analysis of the phytochemical compounds found in the stem of T. cordifolia is studied and FRAP, DPPH and ABTS assay is carried out to analyse the antioxidative activity of the three solvents including aqueous, ethanol and acetone of T. cordifolia stem. Results: In a qualitative analysis of the T. cordifolia stem the compounds carbohydrates, alkaloids, flavonoids, reducing sugar, phenolic compounds, tannins, saponins, phytosterol, lignin, quinone, anthocyanin and coumarins are found present. In FRAP among the three solvent extracts ethanolic extract of T. cordifolia stem performed better followed by acetone and aqueous. In DPPH the aqueous extract of T. cordifolia stem exhibited better antioxidative activity with IC₅₀ 136 μg/mL followed by acetone and ethanol extract. In the ABTS assay too the aqueous extract showed better antioxidative properties with IC₅₀ 48.150 μg/mL followed by acetone and ethanol extract of the plant. Conclusion: The above study indicates that T. cordifolia has medicinal properties due to the existence of different phytochemical constituents. In antioxidative activity analysis, the aqueous extract of the T. cordifolia stem performs better than the other two extracts, which indicates that the aqueous extract has good antioxidative properties. However, further analysis is needed.