The rat ovaries after U-74389G process

Abstract

Aim: This study co-evaluated the 4 quoted histologic variables after U-74389G (L) administration. The calculation was based on the results of 2 preliminary studies, each one evaluating two respective histologic variables of ovarian epithelium edema (OE), oophoritis (OO), ovarian epithelium karyorrhexis (OK), ovarian congestion (OC); in an induced ischemia reperfusion animal experiment. Furthermore, the cytokine (TNFα) and the marker for oxidative stress malondialdehyde (MDA) were also calculated.

Materials and methods: The 2 main experimental endpoints at which the OE, OO and OK, OC and TNFα, MDA scores were evaluated was the 60^th reperfusion min (for the groups A and C) and the 120^th reperfusion min (for the groups B and D). Specially, the groups A and B were processed without drugs, whereas the groups C and D after L administration.

Results: The first preliminary study showed that L has a very significant recessing potency for OE and OC together (p-values=0.0157) within the “without lesions” alterations 0.1772727 [-0.3191054 - -0.03544]. The second preliminary study showed that L had a non-significant recessing potency for OK and OC within the “without lesions” grade 0.1772727 [-0.3716027 - +0.0170573] together (p-values=0.0726).  These 2 studies were co-evaluated since they came from the same experimental setting. This study co-evaluated the combined diagnostic values of the four variables together. Separate calculations were performed for TNFα and MDA scores.

Conclusions: L administration significantly suppressed the 4 histologic variables within the “without lesions alterations” score 0.1772727 [-0.3208232 - -0.0337223] (p-value=0.0169 ), non significantly reduced the TNFα levels by 4.69% [+8.40%] (p-value=0.5749) and significantly reduced the MDA levels by 9.85%+2.66% (p-value=0.0005).