Antioxidant potential and biochemical evaluation of metabolites from the marine bacteria Virgibacillus sp. associated with the sponge Callyspongia diffusa

Authors

  • Kalirajan Arunachalam Department of Zoology, Sri Paramakalyani College, Alwarkurichi 627412, Tirunelveli (DT), Tamil Nadu, India
  • Ranjitsingh Amirtham Jacob Appadorai Department of Zoology, Sri Paramakalyani College, Alwarkurichi 627412, Tirunelveli (DT), Tamil Nadu, India

Keywords:

Antioxidants , Callyspongia diffusa , Marine drug , Virgibacillus sp

Abstract

Objective: In this present research, the antioxidant activity of the metabolites produced by the marine bacteria Virgibacillus sp. associated with the sponge Callyspongia diffusa was evaluated and the crude metabolites was also tested to find out the chemical compounds. Methods: In view of testing the antioxidant activity, DPPH radical scavenging activity, Hydroxyl radical scavenging activity, Superoxide radical scavenging activity, Nitric oxide radical scavenging activity,, Metal chelating, Ferric reducing antioxidant power were carried out in vitro. Results: The DPPH radical scavenging activity was 46.56% at 2000 mg/ml and the IC50 value was 857.49 mg/ ml, The hydroxyl radical scavenging activity was found significant (60.77% at 2 mg/ml) and the IC50 value was found 471.07 mg/ml. Superoxide radical scavenging activity was 25.58% at 2000 mg/ml and the IC50 was 1353.28 mg/ml, for nitric oxide, the radical scavenging activity was 25.58% at 2000 mg/ml concentration and in the due course the IC50 value was 1353.28 mg/ml. The results of the chemical analysis showed the presence of chemical components such as alkaloids, terpenoids, reducing sugars and anthraquinones. Conclusion: This research revealed the potential of marine sponge-associated bacterial bioactive compounds in scavenging the free radicals invitro. Separation of individual compounds from the crude metabolites of Virgibacillus sp. associated with the marine sponge C. diffusa will be resulted in the development of a novel antioxidant molecule.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...
Phylogenetic tree of the strain CSD-5 associated with the margin sponge Callyspongia diffusa.

Downloads

Published

2013-03-29

How to Cite

Kalirajan Arunachalam, & Ranjitsingh Amirtham Jacob Appadorai. (2013). Antioxidant potential and biochemical evaluation of metabolites from the marine bacteria Virgibacillus sp. associated with the sponge Callyspongia diffusa. Free Radicals and Antioxidants, 3(1), 47–51. Retrieved from http://mail.antiox.org/index.php/fra/article/view/233